Degradative Enzymes of Insoluble Glucans Produced by Steptococcus mutans
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چکیده
منابع مشابه
Stimulation of various functions in murine peritoneal macrophages by glucans produced by glucosyltransferases from Streptococcus mutans.
The glucan that was produced by glucosyltransferases (GTFs) from Streptococcus mutans was examined for its stimulating functions toward murine peritoneal macrophages. Soluble glucan was obtained by the reaction with cell-free crude GTFs and sucrose, followed by ethanol precipitation, dispersion in water and re-precipitation by ethanol. Soluble glucan, those average molecular weight was about 3 ...
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The intact gtfC gene from Streptococcus mutans GS-5 was isolated in Escherichia coli in plasmid vector pUC18. The glucosyltransferase activity expressed by the gene synthesized both low-molecular-weight water-soluble glucan and insoluble glucan in a primer-independent manner. Purification of the enzyme by procedures that minimize proteolytic digestion yielded a purified preparation with a molec...
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Streptococcus mutans is considered the principal cariogenic bacterium for dental caries. Despite the recognition of their importance for cariogenesis, the possible coordination among S. mutans' main virulence factors, including glucan production, acidogenicity and aciduricity, has been less well studied. In the present study, using S. mutans strains with surface-displayed pH-sensitive pHluorin,...
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UNLABELLED Glucosyltransferase B is an enzyme produced by Streptococcus mutans, which catalyzes synthesis from sucrose of insoluble glucans that provide support to the biofilm. It is one of the main virulence factors in the generation of dental caries. However, its role is unclear in caries-free individuals who carry the bacteria. The aim of this study was to determine the production of glucosy...
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ژورنال
عنوان ژورنال: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
سال: 1976
ISSN: 0385-0110,1880-408X
DOI: 10.2329/perio.18.32